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KMID : 1149220210290030119
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Korean Journal of Oriental Medical Prescription
2021 Volume.29 No. 3 p.119 ~ p.125
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Modulation of Fermented Lotus Root on Pacemaker Potentials in Interstitial Cells of Cajal of Murine Small Intestine
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Park Dong-Suk
Kim Jung-Nam
Kwon Hyo-Eun
Kwon Min-Ji
Park Eun-Jung
Lee Hae-Jeung
Kim Byung-Joo
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Abstract
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Obejectives : The purpose of this study is to find out the efficacy of pacemaker potentials of interstitial Cells of Cajal (ICC) by Fermented Lotus Root (FLR) in small intestine.
Methods : Enzyme digestions were used to separate the ICC. Using electrophysiological methods, pacemaker potentials were measured and intestinal transit rates (ITR) experiments were conducted to identify in vivo gastrointestinal motility.
Results : 1. FLR (0.5-10 mg/ml) caused membrane depolarization by electrophysiological methods.
2. In the case of pretreatment with a Ca2+ free solution and thapsigargin, the pacemaker potential disappeared and in this case, FLR did not have a membrane depolarization reaction.
3. Lowering the concentration of extracellular Na+ concentration stoped the pacemaker potentials and inhibited the reaction caused by FLR. Flufenamic acid also inhibited the reaction by FLR.
4. In mice, ITR was increased by FLR.
Conclusions : This study shows that FLR can control ICC by an internal/external Ca2+ and Na+. It also shows that FLR can be a good candidate for gastrointestinal motility medication development.
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KEYWORD
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Fermented Lotus Root, Interstitial Cells of Cajal, Gastrointestinal motility, Medication development
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